diff --git a/.github/workflows/run-tests.yml b/.github/workflows/run-tests.yml
index 9325829a0..15753f538 100644
--- a/.github/workflows/run-tests.yml
+++ b/.github/workflows/run-tests.yml
@@ -22,7 +22,6 @@ jobs:
     runs-on: ${{ matrix.os }}
     env:
       NO_ET: 1
-      DANDI_ALLOW_LOCALHOST_URLS: "1"
       DANDI_PAGINATION_DISABLE_FALLBACK: "1"
       DANDI_TESTS_PERSIST_DOCKER_COMPOSE: "1"
     strategy:
diff --git a/tox.ini b/tox.ini
index ffdb9b6e9..16ea23744 100644
--- a/tox.ini
+++ b/tox.ini
@@ -3,7 +3,6 @@ envlist = lint,typing,py3
 
 [testenv]
 setenv =
-    DANDI_ALLOW_LOCALHOST_URLS=1
     DANDI_PAGINATION_DISABLE_FALLBACK=1
 passenv = DANDI_*,USER,DBUS_SESSION_BUS_ADDRESS
 extras =